Anti Diabetic Activity Of Sea Cucumber Essay - 1512 Words

Anti diabetic activity of Sea cucumber, Holothuria thomasi Saponin of Streptozotocin Diabetic Rats ABSTRACT This study was designed to confirm the presence of saponins in the Holothuria thomasi and evaluate its anti-diabetic potential in STZ- induced diabetic rats. The soapy lathers when mixed and agitated with water confirmed the presence of saponin in Holothuria thomasi, TLC showed the presence of one type of saponin, the functional group of saponin was similar to commercial standard saponin this confirmed with FTIR analysis and GC-MS analysis showed that the aglycone part of saponin was methyl esters of octadecanoic acid. Saponin extract was administrated orally to STZ-diabetic rats after 35 days of STZ-induction. Saponin extract significantly reduced serum blood glucose and significantly increased serum insulin level indicating that saponin could be antidiabetic effect. Saponin extract significantly decrease both serum cholesterol and triacylglycerol which indicated that saponin act as antihyperlipidemic. Also, saponin extract showed a significant decrease in both serum IL-6 and TNF-ÃŽ ± which indicated that saponin could be a good inhibition for inflammation. Saponin extract significantly decrease liver L-MDA , catalase and significantly increase liver non protein thiol this confirm that saponin could act as antioxidant. Histological examination of the pancreas of diabetic non treated group showed degenerative change in ÃŽ ²-cells, where as treatment reversed histo